分子信标荧光探针用于抑癌基因ING1表达产物的定量测定

根据抑癌基因ING1基因的序列设计并合成了检测ING1转录产物的分子信标核酸探针,发展了一种快速测量从正常细胞系和鼻咽癌肿瘤细胞系提取的ING1转录产物的方法,所得结果与用逆转录结合PCR法(RT-PCR)得到的结果相吻合.并且,将能表达ING1基因的质粒转入肿瘤细胞进行培养后,再将分子信标转入肿瘤细胞,发现转导了质粒的肿瘤细胞比未转导质粒的肿瘤细胞内的荧光明显增强,从而进一步证实了所设计的分子信标核酸探针与ING1转录产物的结合.     

纳米金属粉对高氯酸铵热分解特性的影响

催化性能;纳米金属粉对高氯酸铵热分解特性的影响     

Study of Signature Inversion in the πi13/2 νi13/2Bands of Odd Odd Nuclei

High-spin states in odd-odd nuclei of ^176,178Ir and ¹⁸²Au have been studied experimentally using heavy-ion- induced fusion-evaporation reactions and standard in-beam γ-ray spectroscopic techniques. Rotational bands built on the πi_13/2 νi_13/2 configuration have been observed. In addition, on the basis of level spacing systematics, spin assignments have been proposed for the πi_13/2 νi_13/2 band in ¹⁸⁴ Au,which was reported without spin assignments in previous study. It is pointed out that the πi_13/2 νi_13/2 bands in ^176,178Ir and ^182,184Au present a low-spin signature inversion. In the theoretical framework of 2-quasiparticle plus rotor model with p- n residual interaction, the signature inversion phenomenon in the πi_13/2 νi_13/2 bands has been discussed qualitatively. Theoretical investigations have been performed for the πh_9/2 νi_13/2 and πi_13/2 νi_13/2 bands using the cranked shell model. It is found that the signature inversions in both configurations can be well reproduced using the pairing and deformation self-consistent cranked Wood-Saxon calculations.     

核物理中的π介子

在强子层次上,原子核或强子物质的基本组元是核子和介子.弄清这些强子的结构,并由基本原理出发研究它们的性质,是当代核物理的重要课题.在各种介子中,π介子是最轻且最重要的介子.关于自由空间中π介子的结构与性质、核介质内π介子的性质、π 核子相互作用与π 核相互作用等问题,始终受到相当多的关注.π介子在核物理中的作用直接联系着手征对称性,汤川秀树关于π介子的最初概念已经大大发展了.有清楚的实验证据表明,核内存在π介子的集体模式,这种集体模式与以前观测到的所有核集体运动模式截然不同.拟对π 核物理的研究现状及值得进一步研究的主要问题予以简要评述. At the hadronic level, nucleons and mesons are constituents of nuclei and hadronic matter. Understanding the structures of hadrons, finding the physics of how the properties of these particles arise from the first principle, are major interests in modern nuclear physics. Among mesons, the lightest and most important one is certainly the pion, thus it is no accident that its structure, properties (both in free space and in nuclear matter) and interactions with nucleons and nuclei have gotten considerable ...     

Synthesis And Biological Activities of 4-Neoalkyl-2,6,7-Trioxa-1-PhosphabicycIo[2.2.2] Octanes

4-Alkyl derivatives of 2,6,7-trioxa-1-phosphabicyclo[2.2.2] octanes are extensively used for spectroscopic studies and are candidates for use as flame retardants, vinyl resin stabilizers and antioxidants^[1-4]. Bicyclic phosphates are antagonist of GABA receptor, further research show that these compounds act on the chloride ion channel connecting with GABA receptor. So bicyclic phosphate can act as drugs to probe the Cl^- channel. Our preliminary pharmacological results indicate that these bicyclic phosphorus compounds should be handled with great care because of their high toxicity to mammals. Some derivatives of bicyclic phosphates have been prepared in our laboratory.     

细胞动力学研究VI. 厌氧菌生长过程热化学特征

用LKB2277生物活性检测系统测定了三种厌氧菌---吉氏类杆菌、艰难梭状芽胞杆菌、黑色消化球菌的生长发热功率曲线。根据广义logistic方程, 建立了细菌生长过程的热动力学方程:ln[P/(Pm-P)^v^+^1]=ln[P0/(Pm-P0)^v^+^1]+k.t。由此热动力学方程, 求得了细菌的生长常数k, 根据Arrhenius公式求得吉氏菌的生长活化能Ea=59.7kj/mol, 应用过渡态理论得到吉氏菌在不同温度下的活化力学参数, 这个热动力学方程描述的是一系列不规则的细菌生长过程发热曲线, 将它与经典的指数模型和logistic模型进行比较, 它具有更广泛的适用性。     

带有长侧链醌类化合物的Langmuir膜及其表面电势

泛醌(Ubiquinone)和质体醌(Plastoquinone)作为非蛋白电子载体存在于动物线粒体呼吸链和植物光合作用链中.这两类物质在生物膜上有活性.     

水合醛缩酶热变性的DSC研究

本文采用差示扫描量热法(DSC),研究了水合醛缩酶在含水量h从0.08g/g至2.28g/g范围内的热变性行为。实验结果表明,当h=0.08g/g时,水合醛缩酶样品的温度扫描热谱图呈现了两个吸热峰,其中低温峰很小,高温峰很大。随着h的增大,低温峰变得更小,高温峰则出现了分峰现象。当h=0.22g/g时,低温峰消失,高温峰则分裂成了两个独立的吸热峰。随着h的继续增大,其中前峰的位置不断移向低温,直至h_0.65g/g时,才不再变化。后峰的位置则始终保持不变。当再进行第二次温度扫描时,前峰消失,而后峰却能再现。本文给出了两峰在不同含水量时的转变温度、转变焓和转变焓之和,以及它们与含水量的关系。最后,对上述诸峰的起因作了分析讨论,认为低温峰可能起因于水合醛缩酶结晶的熔化作用,前峰和后峰可能起因于醛缩酶中两种A亚基的热变性。     

一种合成单黄酮类化合物的简便方法

黄酮类化合物是植物最普通的成分之一,其合成大多采用查尔酮关环、黄烷酮脱氢、Baker-Venkataraman重排、Allan-Robinson缩合等方法,但这些方法操作较繁。近年来,虽有不少改进,但基本上还是沿用老路线。本文报道合成此类化合物的一种简便方法。以α-卤代邻羟基苯乙酮1(或2)与芳香醛3,     

镧系元素硝酸盐与1,8-萘啶氮单氧化物固态配合物的合成及性质研究

合成了组成为Ln(C8H6N2O)2(NO3)3.(Ln=La-Lu)的镧系硝酸盐化合物与1,8-二氮萘-N-单氧化物的固体络合物,用元素分析,UV,IR,摩尔电导,差热分析,热重分析及X光粉末衍射等测定了新化合物的组成及有关性质.